Khase chromatography. menyetla ya mokhoa

Khase chromatography - and analysis mokhoa, fumana e ntle haholo ntshetsopeleng mogopolofela. Hore ho ithuta ka hloko ea metheong ea eona mogopolofela le e sebetsang ea etsa lilemong tse leshome tse fetileng, ho ntshetsa pele e potlakileng ea mokhoa ona.

E o tsejoa hore khase chromatography fapane le mekhoa e meng eo e khase sebelisoa ho eona e le mohato mobile. Mohato ema ka ba o tiileng kapa mokelikeli. Ho itšetlehile ka hore hona e fana ka maikutlo adsorption khase kapa khase-mokelikeli chromatography.

The karohano ya metsoako ea lintho tse ka lintho tse kang ho chromatograph khase, ke ka lebaka la ho pheta-pheta khafetsa tsa tshebetso ya ho aba dikarolo pakeng tsa mokelikeli ho ema kapa mohato tiileng le khase tsamaea. Tsela karohano e thehiloe ho se tšoane ka volatility le solubility tsa analytes. karolo e 'ngoe, eo volatility ka fuoa mocheso khōlō le ka tlaase ho e solubility ya mohato ema tla re susumelletsa hore ka lenaneng la ho feta.

Ho sebelisa khase e le microcyteme phikolosetsa dumella ho fumana menyetla kang karohano ya dintho constituent hlaka le lebelo la hlahlojoe. sampole teko e hlahiswa ka lenaneng la foromo gaseous kapa foromo mouoane. Ka mokhoa ona ho ka etsahala ho sekaseka likhase ha feela empa hape se metsi 'me lintho tse tiileng, eo fetolela ka boemo ba lakatsa ka futhumatsang. Tabeng ena, ka khase chromatography mocheso oo tshebetso kaofela, o phetha karolo ea bohlokoa haholo. meeli tshebetso bakeng sa khase adsorption and analysis mokhoa ranges ho tloha 70 ho ba 600 ° C, 'me bakeng sa khase-mokelikeli - ho tloha 20 ho ea ho 400 ° C. Indasteri ea hlahisa khase chromatograph, eo dumella hore mananeo a mocheso esale pele.

Mokhoa ona o etsa hore ho ka khoneha ho sekaseka le ntho ea limolek'hule boima ka tlase ho 400. Ha ba bola ka mouoane, 'me u se ke ua fetola sebopeho bona ka condensation morago ga moo.

Haholo-holo ka mokhoa katisediwa sebelisa chromatography khase-mokelikeli. Bapisoa le khase adsorption e na le melemo e itseng, tse haholo-holo tse amanang le mefuta e fapaneng mefuta e sa khoneha ema mokelikeli mohato, 'me ho hloka sekoli phahameng' me eo ke ea bohlokoa haholo, le pathomorphology ya sephalli.

Khase chromatography - ka mokhoa potlako, tšoauoa ka ho nepahala e phahameng, nahanela le bokhoni ba ho automate. The versatility le fetoha le maemo tsa mokhoa le sebelisoa ho bona hore taba e itseng sesebediswa. Khase chromatography hore hiriloe bakeng and analysis ditirisanommogo fana diphetho ferekanye, e leng se ka belaela.

Mokhoa ona ka rarollang ngata katisediwa mathata, arola le ho fumana hore na karo-karolelano ea dikgokelo ho bonyane la khatello mouoane. mokhoa o mong oa khase chromatography e sebediswa bakeng sa lik'hemik'hale tlhoekiso, petsoha ka dikarolo motho ea metsoako ena. Ke haholo-holo e atlehang ka karohano ya sebopeho tshwanang ya dintho tseo e matleng a hae ho sehlopheng sona seo: acid manyolo, alcohols, hydrocarbons.